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Recombinant Human Integrin alpha 6 beta 1 Protein (Flag & His Tag)

Recombinant Human Integrin alpha 6 beta 1 Protein (Flag & His Tag)

Item no.	CT013-H2508H-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Neuroscience Metabolism Cholesterol & Lipid Metabolism Aging & Neurological Disorders Neural Adhesion Molecules Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Human Cell
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD49f, ITGA6B, VLA-6
Similar products	ITGA6 & ITGB1
Available
Manufacturer - Category	Protein
Molecule	ITGA6 & ITGB1
Synonym	CD49f, ITGA6B, VLA-6
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ser 1011) of human ITGA6 isoform alpha-6X1B (P23229-3) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the extracellular domain (Met 1-Asp 728) of human ITGB1 (P05556-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
Expressed Host	Human Cells
Purity	> 92 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Phe 24 & Gln 21
Molecule Mass	The recombinant heterodimer of human ITGA6/ITGB1 comprises 1779 (1030 + 749) amino acids and has a calculated molecular mass of 198 (115 + 83) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGA6/ITGB1 heterodimer is approximately 110 & 130 kDa in SDS-PAGE under reducing conditions
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	P23229-3 & P05556-1
Amino acid sequence	Met1-Ser1011 & Met1-Asp728
TAG	C-Flag & C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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