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Recombinant Human IL-35 Protein

Recombinant Human IL-35 Protein

Item no.	CT040-H0323H-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CLMF, IL-12A, NFSK, NKSF1, P35
Available
Manufacturer - Category	Protein
Molecule	IL12A & IL27B
Synonym	CLMF, IL-12A, NFSK, NKSF1, P35
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Expressed Host	Human Cells
Purity	(59.8+33.3) % as determined by SDS-PAGE
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 23 & Arg 21
Molecule Mass	The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Accession	NP_000873.2 & NP_005746.2
Amino acid sequence	Met1-Ser219 & Met1-Lys229
TAG	C-human IgG1-Fc & His & C-human IgG1-Fc & Flag

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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