Recombinant Mouse ACVR2B Protein (His Tag), HPLC-verified

C-His

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the extracellular domain of mouse ACTRIIB (NP_031423.1) (Met 1-Thr 134) was fused with a polyhistidine tag at the C-terminus.

1. Measured by its binding ability in a functional ELISA. Immobilized human ACVR2B (Cat:10229-H02H) at 10 ug/mL (100 ul/well) can bind biotinylated mouse INHBA-His (Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:50659-M08H) is 0.161 ug/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse INHBA-his at 10 ug/mL (100 ul/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 ug/mL.
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 ug/mL in the presence of 10 ng/mL recombinant Activin A.

Ser l9

Lyophilized from sterile PBS, pH 7.4