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Mouse R-Spondin 1 / RSPO1 Protein (His Tag)

Mouse R-Spondin 1 / RSPO1 Protein (His Tag)

Item no.	50316-M08S-20
Manufacturer	Sino Biological
Amount	20 ug
Category	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	CHO Stable Cells
Specific against	Mouse (Murine, Mus musculus)
Host	CHO cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	R-spondin, Rspondin
Similar products	RSPO1
Available
Molecule	RSPO1
Synonym	R-spondin, Rspondin
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	CHO Stable Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ser 21
Molecule Mass	The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_619624.2
Amino acid sequence	Met1-Gln265
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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