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Recombinant Mouse CD40 Ligand/TNFSF5 Protein (hFc Tag), HPLC-verified

Recombinant Mouse CD40 Ligand/TNFSF5 Protein (hFc Tag), HPLC-verified

Item no.	50327-M01H-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug 500 ug
Category	Neuroscience Neural Lineage Markers Aging & Neurological Disorders Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
Conjugate/Tag	Fc
Purity	> 80 % as determined by SDS-PAGE
Sequence	Gly115-Leu260
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD154,CD40-L,Cd40l,gp39,HIGM1,IGM,IMD3,Ly-62,Ly62,T-BAM,Tnfsf5,TRAP
Similar products	CD40LG
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CD40LG
Protein Construction	A DNA sequence encoding the soluble form of mouse CD40LG (P27548) (Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse CD40L-Fc (Cat:50327-M01H) at 10 ug/mL (100 ul/well) can bind biotinylated mouse CD40-Fch (Cat:50324-M03H), The EC50 of biotinylated mouse CD40-Fch (Cat:50324-M03H) is 33-49 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu
Molecule Mass	The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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