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Recombinant Mouse SCF Protein (ECD, His Tag), HPLC-verified

Recombinant Mouse SCF Protein (ECD, His Tag), HPLC-verified

Item no.	50487-M08B-5
Manufacturer	Sino Biological
Amount	5 ug
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 5 ug
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Ala189
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	50487-M08B
Similar products	KITLG
Available
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	KITLG
Protein Construction	A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 ug/ml (100 ul/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.). The EC50 for this effect is typically 0.5-5 ng/ml. 3. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 6-24 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Lys 26
Molecule Mass	The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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