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Recombinant Mouse GSK-3 beta/GSK3B Protein (His Tag)

Recombinant Mouse GSK-3 beta/GSK3B Protein (His Tag)

Item no.	50650-M07B-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Neuroscience Metabolism Cholesterol & Lipid Metabolism Aging & Neurological Disorders Neural Adhesion Molecules Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3
Shipping Condition	Cool pack
Available
Manufacturer - Category	Protein
Shipping method	Blue ice shipping
Molecule	GSK3B
Synonym	7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the mouse GSK3B (Q9WV60) (Met 1-Thr 420) was fused with a polyhistidine tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	1. The specific activity was determined to be 61 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-mGSK3B at 10 ug/ml (100 ul/well) can bind biotinylated human HG3C-CTNNB1 (cat:11279-H20B), EC50 of biotinylated human HG3C-CTNNB1 (cat:11279-H20B) is 0.15-0.35 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant mouse GSK3B consists of 439 amino acids and has a calculated molecular mass of 49 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 25% glycerol, 0.2mM DTT, pH 7.4
Accession	Q9WV60
Amino acid sequence	Met1-Thr420
TAG	N-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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50650-M07B-50-datasheet.pdf

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Amount: 50 ug

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