Comparison

Recombinant Rat CNTF Protein European Partner

Item no. 80012-RNAE-20
Manufacturer Sino Biological
Amount 20 ug
Quantity options 1 mg 100 ug 2 mg 20 ug 4 mg
Category
Type Proteins
Format E.coli
Specific against Rat (Rattus norvegicus)
Host E.coli
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias 80012-RNAE
Similar products CNTF
Available
Manufacturer - Category
Protein
Molecule
CNTF
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
Expressed Host
E.coli
Purity
> 92 % as determined by SDS-PAGE
Bio-Activity
1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 ug/ml (100 ul/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-40 ng/ml.
Endotoxin
Please contact us for more information.
Predicted N Terminal
Met 1
Molecule Mass
The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5
Accession
P20294
Amino acid sequence
Met1-Met200
TAG
Native

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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