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Recombinant Rat Erythropoietin/EPO Protein (His Tag)

Recombinant Rat Erythropoietin/EPO Protein (His Tag)

Item no.	80055-R08B-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	1 mg 2 mg 20 ug 4 mg
Category	Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins Flow Cytometry ELISA
Type	Proteins
Format	Baculovirus-Insect cells
Applications	FC, ELISA
Specific against	Rat (Rattus norvegicus)
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80055-R08B
Similar products	EPO
Available
Manufacturer - Category	Protein
Molecule	EPO
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the rat EPO (P29676-1) (Met 1-Arg 192) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its ability to bind human EPOR-Fc (Cat:10707-H02H) in a functional ELISA. 2. Measured by its ability to bind biotinylated human EPOR-His (Cat:10707-H08H) in a functional ELISA. 3. Measured by its ability to bind mouse EPOR-Fch (Cat:50031-M03H) in a functional ELISA. 4. Measured by its ability to bind human EPOR & CD131-Fc (Cat:CT010-H02H) in a functional ELISA. 5. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 2-10ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ala 27
Molecule Mass	The recombinant rat EPO comprises 176 amino acids with and has a predicted molecular mass of 20 kDa. It migrates as an approximately 27 kDa band in reduced SDS-PAGE.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Accession	P29676-1
Amino acid sequence	Met1-Arg192
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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80055-R08B-20-datasheet.pdf

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Amount: 20 ug

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