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Recombinant Rat CD47 Protein (His Tag), HPLC-verified

Recombinant Rat CD47 Protein (His Tag), HPLC-verified

Item no.	80305-R08H-100
Manufacturer	Sino Biological
Amount	100 ug
Quantity options	1 mg 100 ug 2 mg 4 mg
Category	Neuroscience Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Human Cell
Specific against	Rat (Rattus norvegicus)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80305-R08H
Similar products	CD47
Available
Manufacturer - Category	Protein
Molecule	CD47
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 ug/ml (100 ul/well) can bind mouse SIRPA-Fc (Cat:50956-M02H), The EC50 of mouse SIRPA-Fc (Cat:50956-M02H) is 0.6-1.4 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gln 19
Molecule Mass	The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	P97829-1
Amino acid sequence	Met1-Lys140
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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