Manufacturer |
Cusabio
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Isotype |
IgG |
Format |
Liquid |
Applications |
WB, IF, IHC, ELISA |
Amount |
100ul |
Host |
Rabbit |
Item no. |
CSB-PA258817 |
Targets |
CDK5R2, JUN |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Immunogen Species |
Human |
UniprotID |
P05412 |
Immunogen |
Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun. |
Applications info |
ELISA, WB, IHC, IF, WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Dilution ratio 1 |
ELISA:1:2000-1:10000 |
Dilution ratio 2 |
WB:1:500-1:1000 |
Dilution ratio 3 |
IHC:1:50-1:100 |
Dilution ratio 4 |
IF:1:100-1:200 |
Background |
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Boyle W J, et al. (1991) Cell. 64(3): 573-584. Binetruy B, et al. (1991) Nature. 351: 122-127. Smeal T, et al. (1991) Nature. 354:494-496. Derijard B, et al. (1994) Cell. 76:1025-1037. Kyriakis J M, et al. (1994) Nature. 369: 156-160. |
Conjugate |
Non-conjugated |
Buffer |
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage |
Upon receipt, store at -20C or -80C. Avoid repeated freeze. |
Purification Method |
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
General Research Areas |
Epigenetics and Nuclear Signaling, Cancer, Immunology, Signal transduction |
Initial Research Areas |
Epigenetics and Nuclear Signaling |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.