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Phospho-PRKCZ (Thr560) Antibody

Phospho-PRKCZ (Thr560) Antibody

Manufacturer	Cusabio
Category	Oncology Neuroscience Neural Signaling Antibodies By Cell Type Melanoma Western Blot ELISA Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Isotype	IgG
Format	Liquid
Applications	WB, ELISA
Amount	100ul
Host	Rabbit
Item no.	CSB-PA295272
Targets	PRKCZ
eClass 6.1	32160702
eClass 9.0	32160702
Available
Immunogen Species	Human
UniprotID	Q05513
Immunogen	Peptide sequence around phospho rylation site of threonine560(Q-L-T(p)-P-D) derived from Human PKC zeta .
Applications info	ELISA, WB, WB:1:500-1:1000
Dilution ratio 1	ELISA:1:2000-1:10000
Dilution ratio 2	WB:1:500-1:1000
Background	This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Nakayama M, et al. (2013)Nat Cell Biol 15, 249-60. Mayanglambam A, Bhavanasi D, Vijayan KV, Kunapuli SP (2011)Biochem Pharmacol 82, 505-13. Dettori R, et al. (2009)J Biol Chem 284, 30318-27.
Conjugate	Non-conjugated
Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage	Upon receipt, store at -20C or -80C. Avoid repeated freeze.
Purification Method	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
General Research Areas	Signal transduction
Initial Research Areas	Signal Transduction

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ul

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Delivery expected until 6/13/2024