Anti-DARPP-32

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Protein phosphatase 1 regulatory subunit 1B

Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Cow; Dog; Human; Mouse; Rat