Manufacturer |
Cell Sciences
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human |
Isotype |
IgG |
Applications |
WB |
Amount |
100 ug |
Host |
Rabbit |
Item no. |
CS-CPC133 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Application Notes |
Western Blotting (1-4 ug/ml) and for immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A.. |
Concentration |
0.2 mg/ml |
Cross-Reactivity |
Human, mouse, rat, rabbit, bovine, hamster, and dog |
Description |
Rabbit Anti-Human Calcineurin A Affinity Purified polyclonal antibody. Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death. |
Formulation |
Liquid in PBS + pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only. |
Immunogen |
Synthetic peptide corresponding to residues surrounding amino acid 267 of human Calcineurin A |
Purification |
Biospecific affinity chromatography |
Reconstitution |
The antibody solution should be gently mixed before use. |
Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
Specificity |
Detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.