Comparison

Recombinant Human Diacylglycerol kinase epsilon(DGKE)

Item no. CSB-CF006835HU-100
Manufacturer Cusabio
Amount 100 ug
Quantity options 10 ug 100 ug 20 ug 50 ug
Category
Type Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Conjugate/Tag Myc, SUMO, HIS
Purity Greater than 85% as determined by SDS-PAGE.
Sequence MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVF ITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQ PTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKR FQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMV CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNE KCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLA SKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLN PVQ
Protein Family Eukaryotic diacylglycerol kinase family
Citations Functional specialization of beta-arrestin interactions revealed by proteomic analysis.
Xiao K., McClatchy D.B., Shukla A.K., Zhao Y., Chen M., Shenoy S.K., Yates J.R., Lefkowitz R.J.
Proc. Natl. Acad. Sci. U.S.A. 104:12011-12016(2007)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Diglyceride kinase epsilon
Available
Manufacturer - Conjugate / Tag
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
83.9 kDa
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
General Research Areas
Signal Transduction
Relevance
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Expression Region
1-567aa
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Subcellular Location
Membrane, Multi-pass membrane protein, Membrane, Cytoplasm
Tissue Specificity
Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
Involvement in disease
Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7)
Paythway
OxidativePhosphorylation
Gene Names
DGKE
Sequence Info
Full Length
Organism
Homo sapiens (Human)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

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