Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
10ug |
Item no. |
CSB-CF842761HUa6-10 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research areas |
Neuroscience |
Target / Protein |
CRBN |
Biologically active |
Not Test |
Expression system |
in vitro E.coli expression system |
Species of origin |
Homo sapiens(Human) |
Uniprot ID |
Q96SW2 |
AA Sequence |
MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVED QDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGR TLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQ EVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAE IYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSD GIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPS KPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLY SLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDF SYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIM NKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYA WTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRS ALLPTIPDTEDEISPDKVILCL |
Tag Info |
N-terminal 6xHis-B2M-tagged |
Expression Region |
1-442aa |
Protein length |
Full Length |
MW |
64.5 kDa |
Relevance |
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3 |
References |
"The DNA sequence, annotation and analysis of human chromosome 3."Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. Gibbs R.A.Nature 440:1194-1198(2006) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3. |
Involvement in disease |
Mental retardation, autosomal recessive 2A (MRT2A) |
Subcellular Location |
Cytoplasm, Nucleus, Membrane, Peripheral membrane protein |
Protein Families |
CRBN family |
Tissue Specificity |
Widely expressed. Highly expressed in brain. |
Tag Information |
N-terminal 6xHis-B2M-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.