Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
10ug |
Host |
E.coli |
Item no. |
CSB-EP009553DXU-10 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research Topic |
Neuroscience |
Uniprot ID |
P04773 |
Gene Names |
GLUL |
Organism |
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) |
AA Sequence |
ATSASSHLNKGIKQMYMSLPQGEKVQAMYIWVDGT GEGLRCKTRTLDCEPKCVEELPEWNFDGSSTFQSE SSNSDMYLSPVAMFRDPFRKEPNKLVFCEVFKYNQ KPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLLG TDGHPFGWPSDGFPGPQGLYYCGVGADKAYRRDIM EAHYRACLYAGVKITGTYAEVKHAQWEFQIGPCEG IRMGDHLWVARFILHRVCKDFGVIATFDSKPIPGN WNGAGCHTNFSTKTMREENGLKHIKEAIEKLSKRH RYHIRAYDPKGGLDNARRLTGFHKTSNINDFSAGV ADRSASIRIPRTVGQEKKGYFEARCPSANCDPFAV TEAIVRTCLLNETGDQPFQYKN |
Expression Region |
2-373aa |
Sequence Info |
Full Length of Mature Protein |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
MW |
58.2 kDa |
Alternative Name(s) |
GS Glutamate decarboxylase Glutamate--ammonia ligase |
Relevance |
Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). |
Reference |
"The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster."Hayward B.E., Hussain A., Wilson R.H., Lyons A., Woodcock V., McIntosh B., Harris T.J.R.Nucleic Acids Res. 14:999-1008(1986) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Storage Buffer |
Tris-based buffer, 50% glycerol |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions |
Subcellular Location |
Cytoplasm, Mitochondrion |
Protein Families |
Glutamine synthetase family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.