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Mini Samples ELISA Kit for Nesfatin 1 (NES1)

Mini Samples ELISA Kit for Nesfatin 1 (NES1)

Item no.	MEA242Mu-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	The minimum detectable dose of this kit is typically less than 187.7pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Specificity	Mus musculus (Mouse)
Quantity options
Detection range	493.8-40, 000pg/mL
Organism species	Mus musculus (Mouse)
Sensitivity	The minimum detectable dose of this kit is typically less than 187.7pg/mL
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Competitive Inhibition
Specificity	This assay has high sensitivity and excellent specificity for detection of Mini Samples Nesfatin 1 (NES1). No significant cross-reactivity or interference between Mini Samples Nesfatin 1 (NES1) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Nesfatin 1 (NES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Nesfatin 1 (NES1) and unlabeled Mini Samples Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.