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ELISA Kit for Interferon Gamma (IFNg)

ELISA Kit for Interferon Gamma (IFNg)

Item no.	SEA049Ga-24T
Manufacturer	Cloud-Clone
Amount	24 T
Quantity options	10x96 T 24 T 48 T 5x96 T 96 T
Category	ELISA & Immunoassays ELISA Kits
Type	Elisa-Kit
Specific against	Chicken (Gallus gallus domesticus)
Sensitivity	The minimum detectable dose of this kit is typically less than 5.5pg/mL
Citations	Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineImmunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusImmune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against CoccidiosisPossible Association of IFN-γ GeneEvaluation of two strains of Marek's disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus.Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.EVALUATION OF HUMORAL AND CELLULAR IMMUNE RESPONSES TO EIMERIA TENELLA OOCYST PROTEIN AS VACCINE TO BROILEREffects of dietary mixture of garlic (Allium sativum), coriander (Coriandrum sativum) andprobiotics on immune responses and caecal counts in young laying hens.Stress Induced Polarization of Immune-Neuroendocrine Phenotypes in Gallus gallusA bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccineImmunogenicity and protective efficacy of recombinant fiber-2 protein in protecting SPF chickens against fowl adenovirus 4Construction of Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific …Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in ChickensAtmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymusEctopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMTImmunological Effects of a Single Dose of PLGA Nanoparticles Encapsulated Peptide in Broilers in Comparison to Traditional Vaccines against Infectious Bursal …Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious?¡Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent …Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IFN-G,IFG,IFI,INFr,IFN,Immune Interferon
Available
Specificity	Chicken (Gallus)
Manufacturer - Applications	Enzyme-linked immunosorbent assay for Antigen Detection.
Manufacturer - Category	ELISA CLIA Kits
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length	3h
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg). No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50uL Stop Solution. Read at 450nm immediately.
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma (IFNg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma (IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Manufacturer - Research Area	Cytokine; Infection immunity

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.