Recombinant Human IL-1 beta Protein, HPLC-verified

Protein

IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

E.coli

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 ug/mL (100ul/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.
3. Measured by its binding ability in a functional ELISA.
4. Immobilized human IL1B at 10 ug/mL (100 ul/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 ug/mL.
5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

Met

Lyophilized from sterile PBS, pH 7.4

Ala117-Ser269