Recombinant Human IL-1 beta Protein, HPLC-verified

Native

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mature form of human IL1beta (NP_000567.1) (Ala 117-Ser 269) was expressed.

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 ug/mL (100ul/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.
3. Measured by its binding ability in a functional ELISA.
4. Immobilized human IL1B at 10 ug/mL (100 ul/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 ug/mL.
5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

Met

Lyophilized from sterile PBS, pH 7.4