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Recombinant Human CTLA4 Protein, Biotinylated

Recombinant Human CTLA4 Protein, Biotinylated

Item no.	230-30054B-200
Manufacturer	Raybiotech
Amount	200 ug
Quantity options	10 ug 200 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	Biotin, HIS
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping condition	Room temperature
Available
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the abundant glycosylation, it migrates as approximately 18-22 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Gene Symbols	CD152CTLA4
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region	Lys36-Asp161
Protein Name & Synonyms	Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152)
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit (lot specific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Preservative	None

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.