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Recombinant Human CTLA4, Biotinylated

Recombinant Human CTLA4, Biotinylated

Item no.	230-30167B-10
Manufacturer	Raybiotech
Amount	10 ug
Quantity options	10 ug 200 ug 50 ug 10 ug 100 ug 200 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	Biotin, HIS
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping condition	Cool pack
Available
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Storage Conditions	Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Molecular Weight	Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the abundant glycosylation, it migrates as approximately 18-22 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Gene Symbols	CD152CTLA4
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region	Lys36-Asp161
Protein Name & Synonyms	Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152)
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit (lot specific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Preservative	None

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10 ug

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