Recombinant Human 14-3-3 Protein Gamma

Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

N-terminal histidine tag

-20°C

Recombinant human 14-3-3 protein gamma with an N-terminal His-tag, derived from the transfected human HEK293 cells.

YWHAG

Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose

Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Gamma, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase, Activation Protein, Gamma Polypeptide, Protein Kinase C Inhibitor Protein 1, 14-3-3 Gamma.

His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.