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Recombinant Mouse PCSK9 Protein (His Tag), HPLC-verified

Recombinant Mouse PCSK9 Protein (His Tag), HPLC-verified

Item no.	50251-M08H-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 10 ug 100 ug 2 mg 20 ug 4 mg
Category	Neuroscience Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins Obesity Lipoprotein Receptors & Associated Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Gln694
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AI415265,AI747682,FH3,HCHOLA3,Narc1,PC9
Similar products	PCSK9
Shipping Condition	Cool pack
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	Blue ice shipping
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	PCSK9
Protein Construction	A DNA sequence encoding the full length of mouse PCSK9 (NP_705793.1) precursor (Met 1-Gln 694) was expressed, with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gln 35
Molecule Mass	The secreted recombinant mouse PCSK9 consists of 671 amino acids and has a calculated molecular mass of 72.6 kDa. As a result of proteolysis and glycosylation, the recombinant protein migrates as doublet with apparent molecular mass of approximately 19 & 65 kDa, cprresponding to the prodomain and the mature form of PCSK9 respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 15mM Tris, 90mM NaCl, 50% Glycerol, pH 7.5

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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