Recombinant Human EGF Protein

< 0.1 EU/μg of the protein by LAL method.

ice pack

33.01 kDa

Recombinant Human EGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn 971-Arg 1023) of human EGF (Accession #NP_001954.2) fused with hFc, 6×His tag at the C-terminus.

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

C-hFc&His

Growth Factor, Cell Culture related

1. Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells.1-10 ng/mL of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation.|2. Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 μg/mL (100 μL/well) can bind Human EGF with a linear range of 7-25 ng/mL. 3. Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 0. 065-0. 26 ng/mL, corresponding to a specific activity of 3. 84×10⁶-1. 54×10⁷units/mg.

33.01 kDa