« Back
Home /
Human Cytokine Array C1000

Human Cytokine Array C1000

Manufacturer	Raybiotech
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Senescence Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Testicular & Prostate Cancer Renal Cancer Lymphatic System Cancer Glial Cell Markers Neuronal Cell Markers Antibody Arrays
Type	Array
Specific against	Human
Amount	8 Sample Kit
Item no.	AAH-CYT-1000-8
Targets	ANG ;; IL2 ;; IL4 ;; IL5 ;; IL6 ;; LEP ;; OSM ;; PGF ;;ADIPOQ;;ANGPT2;;AREG;;AXIN1;;BMP4;;BMP6;;CCL11;;CCL2;;CCL5;;CCL8;;CXCL6;;CXCL8;;FGF4;;FGF6;;FGF7;;FGF9;;FLT3;;ICAM1;;IGFBP1;;IGFBP2;;IGFBP3;;IL10;;IL11;;IL13;;IL15;;IL17A;;IL1A;;IL6ST;;NECTIN1;;NTF4;;
eClass 6.1	32161000
eClass 9.0	32161000
Available
Number of Targets Detected	120
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	ADIPOQ \| AGRP \| ANG \| ANGPT2 \| AREG \| AXL \| BDNF \| BMP4 \| BMP6 \| BTC\| CCL1 \| CCL11 \| CCL13 \| CCL15 \| CCL16 \| CCL17 \| CCL18 \| CCL19 \| CCL2 \| CCL20 \| CCL22 \| CCL23 \| CCL24 \| CCL25 \| CCL26 \| CCL27 \| CCL28 \| CCL3 \| CCL4 \| CCL5 \| CCL7 \| CCL8 \| CNTF \| CSF1 \| CSF2 \| CSF3 \| CX3CL1 \| CXCL1 \| CXCL11 \| CXCL12 \| CXCL13 \| CXCL2 \| CXCL3 \| CXCL5 \| CXCL6 \| CXCL8 \| CXCL9 \| EGF \| EGFR \| FAS \| FGF2 \| FGF4 \| FGF6 \| FGF7 \| FGF9 \| FIGF \| FLT3LG \| GDNF \| HGF \| ICAM1 \| ICAM3 \| IFNG \| IGF1 \| IGF1R \| IGFBP1 \| IGFBP2 \| IGFBP3 \| IGFBP4 \| IGFBP6 \| IL10 \| IL11 \| IL12A \| IL12B \| IL13 \| IL15 \| IL16 \| IL17A \| IL1A \| IL1B \| IL1R1 \| IL1RL1 \| IL1RN \| IL2 \| IL2RA \| IL3 \| IL4 \| IL5 \| IL6 \| IL6R \| IL6ST \| IL7 \| KITLG \| LEP \| LTA \| MIF \| MST1 \| NGF \| NTF3 \| NTF4 \| OSM \| PDGFB \| PGF \| PLAUR \| PPBP \| TGFB1 \| TGFB3 \| THPO \| TIMP1 \| TIMP2 \| TNF \| TNFRSF10C \| TNFRSF10D \| TNFRSF11B \| TNFRSF18 \| TNFRSF1A \| TNFRSF1B \| TNFSF14 \| TNFSF18 \| TPO \| TYRO3 \| VEGFA \| XCL1
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human Cytokine Antibody Array C1000 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer 8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.