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Human ST2/IL-1 RL1 Matched ELISA Antibody Pair Set

Human ST2/IL-1 RL1 Matched ELISA Antibody Pair Set

Item no.	SEK10105-5
Manufacturer	Sino Biological
Amount	5 Plates
Quantity options	15 Plates 5 Plates
Category	Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Type	ELISA Pair Set
Applications	ELISA
Specific against	Human (Homo sapiens)
Conjugate/Tag	HRP
Sensitivity	The minimum detectable dose of human IL1RL1 was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10105
Similar products	IL1RL1
Available
Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	IL1RL1
Capture Ab	1.0 mg/mL of mouse anti-IL1RL1 monoclonal antibody. Dilute to a working concentration of 2.0 ug/mL in CBS before coating.
Detection Ab	0.5 mg/mL of mouse anti-IL1RL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 45 ng of recombinant IL1RL1. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.
Principle of the test	The human IL1RL1 ELISA Pair Set is for the quantitative determinationof human IL1RL1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL1RL1 ( DER4 / ST2) coated on a 96-well plate. Standards and samples are added to thewells, and any IL1RL1 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated mouse anti-IL1RL1 monoclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of IL1RL1 present in the sample. To end theenzyme reaction, the stop solution is added and absorbances of the microwellare read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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