« Back
Home /
Human NBL1 Matched ELISA Antibody Pair Set

Human NBL1 Matched ELISA Antibody Pair Set

Item no.	SEK10169-15
Manufacturer	Sino Biological
Amount	15 Plates
Quantity options	15 Plates 5 Plates
Category	Antibodies ELISA Immunoassay Immunostaining Antibody Pairs
Type	ELISA Pair Set
Applications	ELISA
Specific against	Human (Homo sapiens)
Conjugate/Tag	HRP
Sensitivity	The minimum detectable dose of Human NBL1 / DAND1 / DAN was determined to be approximately 46.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SEK10169
Similar products	NBL1
Available
Manufacturer - Type	ELISA
Storage Conditions	Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.
Antigen molecule name	NBL1
Capture Ab	0.5 mg/mL of mouse anti-DAN monoclonal antibody, Dilute to a working concentration of 2 ug/mL in CBS before coating.
Detection Ab	0.5 mg/mL mouse anti-DAN monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 ug/mL in detection antibody dilution buffer before use.
Standard	Each vial contains 110 ng of recombinant DAN. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
Principle of the test	The Human NBL1 / DAND1 / DAN ELISA Pair Set is for the quantitative determination of Human NBL1 / DAND1 / DAN. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for NBL1 / DAND1 / DAN coated on a 96-well plate. Standards and samples are added to the wells, and any NBL1 / DAND1 / DAN present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-NBL1 / DAND1 / DAN monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of NBL1 / DAND1 / DAN present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

SEK10169-15-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 15 Plates

available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question