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Recombinant Human CDK1 & CCNE1 Heterodimer Protein (His & GST Tag)

Recombinant Human CDK1 & CCNE1 Heterodimer Protein (His & GST Tag)

Item no.	CT027-H0907B-50
Manufacturer	Sino Biological
Amount	50 ug
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Oncology By Cell Type Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Apoptosis Senescence By Organ Lung Cancer Colorectal Cancer Testicular & Prostate Cancer
Type	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CCNE, CDC2, CDC28A, CDK1, CDKN1, P34CDC2 & CCNE1
Available
Manufacturer - Category	Protein
Molecule	CDC2 & CCNE1
Synonym	CCNE, CDC2, CDC28A, CDK1, CDKN1, P34CDC2 & CCNE1
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.
Expressed Host	Baculovirus-Insect cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	The specific activity was determined to be 150 nmol/min/mg using Histone H1 as substrate.
Endotoxin	<1.0 EU per ug protein as determined by the LAL method.
Predicted N Terminal	Met & His
Molecule Mass	The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
Accession	NP_001777.1 & NP_001229.1
Amino acid sequence	Met1-Met297 & Met1-Ala410
TAG	N-GST & N-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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