Manufacturer |
St John's Laboratory
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Isotype |
IgG |
Applications |
WB, IF |
Amount |
50ul |
Host |
Rabbit |
Item no. |
STJ114190-50 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Clonality |
Polyclonal |
Conjugation |
Unconjugated |
Formulation |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification |
Affinity purification |
Uniprot ID |
P15692 |
Storage |
Store at-20° C and avoid freeze-thaw cycles. |
Description |
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. |
Gene ID |
|
Gene symbol |
|
Immunogen |
Recombinant protein of human VEGFA |
Short Description |
Rabbit polyclonal against VEGFA |
Tissue specificity |
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. |
Cellular localization |
SecretedVegf121 Is Acidic And Freely SecretedVegf165 Is More BasicHas Heparin-Binding Properties AndAlthough A Significant Proportion Remains Cell-AssociatedMost Is Freely SecretedVegf189 Is Very BasicIt Is Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
Molecular weight |
Calculated: 15-27kDa/34-45kDa. Observed: 42kDa |
Dilution range |
WB 1:500-1:2000 IF 1:50-1:200 |
Alternative names |
Anti-Vascular Endothelial Growth Factor A antibodyAnti-Vegf-A antibodyAnti-Vascular Permeability Factor antibodyAnti-Vpf antibodyAnti-VEGFA VEGF antibody |
Protein name |
Vascular Endothelial Growth Factor AVegf-AVascular Permeability FactorVpf |
Note |
FOR RESEARCH USE ONLY (RUO). |
Swiss port key |
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Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.