Comparison

Recombinant Human Mannose-6-phosphate isomerase(MPI)

Item no. CSB-EP014754HU-100
Manufacturer Cusabio
Amount 100ug
Quantity options 1mg 10ug 100ug 20ug 200ug 50ug 500ug
Category
Type Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Conjugate/Tag GST
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Phosphohexomutase,Phosphomannose isomerase
Available
Research Areas
Signal Transduction
Uniprot ID
P34949
Gene Names
MPI
Organism
Homo sapiens (Human)
AA Sequence
AAPRVFPLSCAVQQYAWGKMGSNSEVARLLASSDP LAQIAEDKPYAELWMGTHPRGDAKILDNRISQKTL SQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETP LSIQAHPNKELAEKLHLQAPQHYPDANHKPEMAIA LTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAA THLKQTMSHDSQAVASSLQSCFSHLMKSEKKVVVE QLNLLVKRISQQAAAGNNMEDIFGELLLQLHQQYP GDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKG DCVECMACSDNTVRAGLTPKFIDVPTLCEMLSYTP SSSKDRLFLPTRSQEDPYLSIYDPPVPDFTIMKTE VPGSVTEYKVLALDSASILLMVQGTVIASTPTTQT PIPLQRGGVLFIGANESVSLKLTEPKDLLIFRACC LL
Expression Region
1-423aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
73.5 kDa
Alternative Name(s)
Phosphohexomutase
Phosphomannose isomerase
Relevance
Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Reference
Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib).
Schollen E., Dorland L., de Koning T.J., Van Diggelen O.P., Huijmans J.G.M., Marquardt T., Babovic-Vuksanovic D., Patterson M., Imtiaz F., Winchester B., Adamowicz M., Pronicka E., Freeze H., Matthijs G.
Hum. Mutat. 16:247-252(2000)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
Involvement in disease
Congenital disorder of glycosylation 1B (CDG1B)
Subcellular Location
Cytoplasm
Protein Families
Mannose-6-phosphate isomerase type 1 family
Tissue Specificity
Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
Tag Information
N-terminal GST-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
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