Manufacturer |
Cusabio
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid or Lyophilized powder |
Amount |
1mg |
Host |
E.coli |
Item no. |
CSB-EP018489HU-1 |
Conjugate/Tag |
GST |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Research Areas |
Signal Transduction |
Uniprot ID |
P35813 |
Gene Names |
PPM1A |
Organism |
Homo sapiens (Human) |
AA Sequence |
GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEM EDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYC CEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFL EIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYF INCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQ NAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPT EQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMG NEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSR DNMSVILICFPNAPKVSPEAVKKEAELDKYLECRV EEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGEL ASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW |
Expression Region |
1-382aa |
Sequence Info |
Full Length |
Source |
E.coli |
Tag Info |
N-terminal GST-tagged |
MW |
69.3 kDa |
Alternative Name(s) |
Protein phosphatase 2C isoform alpha |
Relevance |
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
Reference |
Protein phosphatase 2Calpha inhibits the human stress-responsive p38 and JNK MAPK pathways. Takekawa M., Maeda T., Saito H. EMBO J. 17:4744-4752(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
Subcellular Location |
Nucleus, Cytoplasm, cytosol, Membrane, Lipid-anchor |
Protein Families |
PP2C family |
Paythway |
MAPKsignalingpathway |
Tag Information |
N-terminal GST-tagged |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.