Comparison

Recombinant Human Proliferation marker protein Ki-67(MKI67),partial

Manufacturer Cusabio
Category
Type Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Amount 100ug
Host E.coli
Item no. CSB-EP014597HU(A5)-100
eClass 6.1 34160400
eClass 9.0 42020190
Available
Research Areas
Cancer
Uniprot ID
P46013
Gene Names
MKI67
Organism
Homo sapiens (Human)
AA Sequence
TKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADK EPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRA PRESAQAIEDLAGFKDPAAGHTEESMTDDKTTKIP CKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVG KLTQTSGETTHTDKEPVGEGKGTKAFKQPAKRKLD AEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPG HTEELANGAADSFTSAPKQTPDSGKPLKISRRVLR APKVEPVGDVVSTRDPVKSQSKSNTSLPPLPFKRG GGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVA PRARGKSSEPVVIMKRSLRTSAKRIEPAEELNSND MKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQ ITEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDG ARKPIPRDKVTENKRCLRSARQNESSQPKVAEESG GQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQP AASTLESKSVQRVTRSVKRCAENPKKAEDNVCVKK IRTRSHRDSEDI
Expression Region
2720-3256aa
Sequence Info
Partial
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
75.1 kDa
Relevance
Thought to be required for maintaining cell proliferation.
Reference
The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins.Schlueter C., Duchrow M., Wohlenberg C., Becker M.H.G., Key G., Flad H.-D., Gerdes J.J. Cell Biol. 123:513-522(1993)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly
Subcellular Location
Chromosome, Nucleus, Nucleus, nucleolus
Tag Information
N-terminal 6xHis-SUMO-tagged

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ug
Available: In stock
available

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